When the Ramsey home was searched, no roll of duct tape matching the tape on JonBenét’s mouth could be found anywhere in the house. Both ends of the duct tape placed over JonBenét’s mouth were torn, not cut.
Same with the parachute cord. The parents didn’t own any parachute cord and none could be found in the house. One end of the cord had been burned to keep it from fraying, while the other end had been cut with a sharp edged instrument and that end was not burned and had begun to fray. The parachute cord had been skillfully used to fashion the garrote and bind JonBenét’s wrists in such a way that the more she struggled, the tighter the knots became.
The stun gun marks on JonBenét’s back are red in color, rectangular in shape, the width is greater than the height, and measure 3.5 cm between the two probes. Lou’s research established the dimensions and distances between the two probes are consistent with an Air Tazer stun gun.
The Hi-Tec boot print did not match any of the footwear found in the Ramsey home and did not match any of the police officers or crime lab personnel. The location of the footprint, on the basement floor, next to the victim’s body, suggests these boots may have been worn by the killer.
All of these items were introduced to the crime scene by the suspect and go to prove the killer’s intent to immobilize the victim with the stun gun, cover her mouth with the duct tape to silence her screams, bind her with the parachute cord and remove her from the home.
The killer also left a ransom note demanding $118,000 to be paid for her return. While some people theorize Patsy authored the note, based in part because the notepaper and pen used to write the ransom note came from a desk inside the home often used by the mother. However, Lou's investigation revealed that a document analyst for the U.S. Secret Service, after an extensive examination of the ransom note, concluded, that there is "no evidence to indicate that Patsy Ramsey" had written the note.
After 25 years it is unlikely items 1-4 will be matched to similar items found in the suspect’s possession; however, the most critical piece of physical evidence recovered from the crime scene, the one piece of evidence which cannot be altered or discarded, is the unknown male DNA found under JonBenét’s fingernails and on the crotch of her panties.
In 2008 the long johns JonBenét was wearing as pajama bottoms the night she was murdered, produced another DNA sample which was also determined to be that of an unknown male, likely Caucasian, and was proven to be consistent with the DNA found under JonBenét’s fingernails and on her panties. This male DNA eliminates all members of the Ramsey family and remains today the strongest evidence to identify the killer. With the advancement in DNA technology, coupled with the exponential growth of DNA databases, there has never been a better time than today to solve the murder of JonBenét Ramsey.